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DSS诱导大鼠溃疡性结肠炎模型

作者:翌圣生物科技(上海)股份有限公司 2024-07-12T00:00 (访问量:25762)

葡聚糖硫酸钠盐(Dextran Sulfate Sodium Salt, DSS)是一种聚阴离子衍生物,其诱导结肠炎模型的机制虽仍未十分明确,但通常认为与巨噬细胞功能失调、肠道菌群失调、DSS对结肠上皮的毒性作用、细胞因子在DSS结肠炎模型的发病中起重要作用等机制有关。

 

 

实验案例1

实验动物:大鼠,1周龄,100 g;

实验步骤:5%的DSS,3.5 mL/100 g每日灌胃,灌胃2周,HE染色;

实验结果:UC大鼠的结肠长度低于健康大鼠;结肠黏膜异常,存在大量炎症细胞。

图1. (a)健康大鼠和UC大鼠的肠道长度;(b)结肠炎切片HE染色结果[1]

 

 

实验案例2

实验动物:大鼠,8周龄,200 g;

实验步骤:饮用3.5% 浓度的DSS,连续饮用10天,HE染色;

实验结果:结肠黏膜异常。

 

图2. 健康大鼠和UC大鼠结肠炎切片HE染色结果[2]

 

 

实验案例3

实验动物:大鼠,7周龄,300-320 g;

实验步骤:饮用7天 2.0 % 浓度的DSS后,饮用7天蒸馏水,共4个周期,HE染色;

实验结果:结肠黏膜异常。

 

图3. UC大鼠造模方法和结肠炎切片HE染色结果[3]

 

 

翌圣DSS产品优势

翌圣生物提供高品质DSS(Cat.NO:60316ES,MW:36000~50000),具有以下优势:

1. 高纯度(>98%),硫含量17-19%,游离硫<0.2%,大量数据文献支持,广泛应用于UC模型的构建。

2. 多种属动物中均可造模:小鼠、大鼠、斑马鱼、猪、果蝇;

3. 症状表现与人UC极度相似:可用于研究急、慢性结肠炎的发生发展机制,也可用于药物的药效研究。

4. 简单易行,成模率高,重复性强

5. 联合氧化偶氮甲烷 (azoxymethane, AOM)用药,可用于诱发结肠炎相关性癌症 (colitis associated cancer, CAC) 动物模型,成功模拟IBD诱发CAC的过程。 

建模效率与进口产品一致,产品价格低至进口价格的1/3,大量现货库存。

 

产品订购

产品名称

货号

规格

价格(元)

促销价(元)

Dextran Sulfate Sodium Salt(DSS)
结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES25

25 g

1255

955

Dextran Sulfate Sodium Salt(DSS)
结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES60

100 g

3855

2655

Dextran Sulfate Sodium Salt(DSS)
结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES76

500 g

16855

9655

Dextran Sulfate Sodium Salt(DSS)
结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

60316ES80

1 kg

32855

18655

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近几年客户使用翌圣DSS已发表文献(部分)

 

向下滑动查看

2024

[1] Zhong D, Jin K, Wang R, ,et al. Microalgae-Based Hydrogel for Inflammatory Bowel Disease and Its Associated Anxiety and Depression. Adv Mater. 2024 Jan 26:e2312275. doi: 10.1002/adma.202312275. IF=29.4

[2] Xuan Zhang, Xia Gao, Xiangzhou Yi,et al. Multi-targeting inulin-based nanoparticles with cannabidiol for effective prevention of ulcerative colitis[J]. Materials Today Bio.2024, 25100965. IF=8.2

 

2022

[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717

[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143

[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451

[4] Wang S,  Huang J,  Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076

[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869

 

2021

[1] Hao H,  Zhang X,  Tong L,  Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From  Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561

[2] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717

[3] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943

[4] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423

[5] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561

 

2020

[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561

[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048

[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245

   

参考文献

[1] Zhou L, Yan F, Jiang R, et al. Administration of Nrf-2-Modified Hair-Follicle MSCs Ameliorates DSS-Induced Ulcerative Colitis in Rats[J]. Oxid Med Cell Longev.

[2] Li B, Du P, Du Y, et al. Luteolin alleviates inflammation and modulates gut microbiota in ulcerative colitis rats[J]. Life Sci.

[3] Song G, Gan Q, Qi W, et al. Fructose Stimulated Colonic Arginine and Proline Metabolism Dysbiosis, Altered Microbiota and Aggravated Intestinal Barrier Dysfunction in DSS-Induced Colitis Rats. Nutrients.

 

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